序列操作套件 SMS2:
引物图谱
绘制引物在序列上的退火位置图谱。
载入示例数据
填充示例序列,方便快速体验
Paste a raw sequence or one or more FASTA sequences into the text area below. Input limit is 200,000,000 characters.
>sample sequence cagctggggggaggtggcgaggaagatgacgtggtcgaggtcgacggtatcgagttgtcgcggcagctgccaatacgactcactatagaggagaagtagcaagaaaaataacatgataattatcacgacaactacctggtgatgttgctagtaatattacttgttatttttctcgtcatcttcccggcgacgtcgccagcaacatctttagtgagggttaatcacctgctacttctcccgccacctccc
Enter the primers you want to display. An example primer entry is:
(T7) aatacgactcactatag
. The round brackets surround the name of the primer, and the sequence is written in the 5' to 3' direction. Multiple entries are separated by commas.
(reverse) aacagctatgaccatg, (T3) attaaccctcactaaag, (KS) cgaggtcgacggtatcg, (SK) tctagaactagtggatc, (T7) aatacgactcactatag, (-40) gttttcccagtcacgac, (Sp6) atttaggtgacactatag, (M13 for) gtaaaacgacggccagt, (M13 rev) cacacaggaaacagctatgaccat, (BGH rev) tagaaggcacagtcgagg, (pGEX for) ctggcaagccacgtttggtg, (pGEX rev) ggagctgcatgtgtcagagg, (T7-EEV aaggctagagtacttaatacga, (pUC/M13 Forward) gttttcccagtcacgac, (pUC/M13 forward) cgccagggttttcccagtcacgac, (pUC/M13 reverse) caggaaacagctatgac, (pUC/M13 reverse) tcacacaggaaacagctatgac, (Glprimer1) tgtatcttatggtactgtaactg, (GLprimer2) ctttatgtttttggcgtcttcca, (RVprimer3) ctagcaaaataggctgtccc, (RVprimer4) gacgatagtcatgccccgcg, (Lambda gt11 Forward) ggtggcgacgactcctggagcccg, (Lambda gt11 Reverse) ttgacaccagaccaactggtaatg, (Lambda gt10 Forward) cttttgagcaagttcagcctggttaag, (lambda gt10 Reverse) gaggtggcttatgagtatttcttccagggta, (Pinpoint Sequencing) cgtgacgcggtgcagggcg, (pTarget Sequencing) ttacgccaagttatttaggtgaca
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vertebrate mitochondrial (2)
yeast mitochondrial (3)
mold mitochondrial (4)
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echinoderm mitochondrial (9)
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bacterial (11)
alternative yeast nuclear (12)
ascidian mitochondrial (13)
flatworm mitochondrial (14)
Blepharisma macronuclear (15)
chlorophycean mitochondrial (16)
trematode mitochondrial (21)
Scenedesmus obliquus mitochondrial (22)
Thraustochytrium mitochondrial (23)
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