序列操作套件 SMS2：
酶切位点突变
寻找可通过突变引入目标酶切位点的区域。
载入示例数据
填充示例序列，方便快速体验
Paste a raw sequence or one or more FASTA sequences into the text area below. Input limit is 10,000,000 characters.
>CDS1 atgtctgattcgctaaatcatccatcgagttctacggtgcatgcagatgatggattcgag ccaccaacatctccggaagacaacaacaaaaaaccgtctttagaacaaattaaacaggaa agagaagcgttgtttacggatctattcgcagatcgtcgacgaagcgctcgttctgtgatt gaagaagctttccaaaacgaactcatgagtgctgaaccagtccagccaaacgtgccgaat ccacattcgattcccattcgtttccgtcatcaaccagttgctggacctgctcatgatgtt ttcggagacgcggtgcattcaatttttcaaaaaataatgtccagaggagtgaacgcggat tatagtcattggatgtcatattggatcgcgttgggaatcgacaaaaaaacacaaatgaac tatcatatgaaaccgttttgcaaagatacttatgcaactgaaggctccttagaagcgaaa caaacatttactgataaaatcaggtcagctgttgaggaaattatctggaagtccgctgaa tattgtgatattcttagcgagaagtggacaggaattcatgtgtcggccgaccaactgaaa ggtcaaagaaataagcaagaagatcgttttgtggcttatccaaatggacaatacatgaat cgtggacagagtgacatttcacttcttgcggtgttcgatgggcatggcggacacgagtgc tctcaatatgcagctgctcatttctgggaagcatggtccgatgctcaacatcatcattca caagatatgaaacttgacgaactcctagaaaaggctctagaaacattggacgaaagaatg acagtcagaagtgttcgagaatcttggaaaggtggaaccactgctgtctgctgtgctgtt gatttgaacactaatcaaatcgcatttgcctggcttggagattcaccaggttacatcatg tcaaacttggagttccgcaaattcactactgaacactccccgtctgacccggaggaatgt cgacgagtcgaagaagtcggtggccagatttttgtgatcggtggtgagctccgtgtgaat ggagtactcaacctgacgcgagcactaggagacgtacctggaagaccaatgatatccaac aaacctgataccttactgaagacgatcgaacctgcggattatcttgttttgttggcctgt gacgggatttctgacgtcttcaacactagtgatttgtacaatttggttcaggcttttgtc aatgaatatgacgtagaagattatcacgaacttgcacgctacatttgcaatcaagcagtt tcagctggaagtgctgacaatgtgacagtagttataggtttcctccgtccaccagaagac gtttggcgtgtaatgaaaacagactcggatgatgaagagagcgagctcgaggaagaagat gacaatgaatag
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AatI agg|cct
AatII gacgt|c
Acc16I tgc|gca
AccII cg|cg
AccIII t|ccgga
AclI aa|cgtt
AcvI cac|gtg
AfaI gt|ac
AfeI agc|gct
AflII c|ttaag
AgeI a|ccggt
AhlI a|ctagt
Alw441 g|tgcac
AluI ag|ct
Aor51HI agc|gct
ApaI gggcc|c
ApaLI g|tgcac
AscI gg|cgcgcc
AseI at|taat
Asp718I g|gtacc
AsuII tt|cgaa
AvaI c|ycgrg
AviII tgc|gca
AvrII c|ctagg
BalI tgg|cca
BamHI g|gatcc
BanIII at|cgat
BbeI ggcgc|c
BbrPI cac|gtg
BbuI gcatg|c
BcuI a|ctagt
BclI t|gatca
BfaI c|tag
BfrI c|ttaag
BfrBI atg|cat
BglII a|gatct
BlnI c|ctagg
BseCI at|cgat
BsePI g|cgcgc
BseX3I c|ggccg
BshTI a|ccggt
Bsp1407I t|gtaca
Bsp19I c|catgg
BspDI at|cgat
BspEI t|ccgga
BsrGI t|gtaca
BssHII g|cgcgc
BstUI cg|cg
ClaI at|cgat
DraI ttt|aaa
EagI c|ggccg
EcoRI g|aattc
EcoRV gat|atc
EgeI ggc|gcc
FseI ggccgg|cc
FspI tgc|gca
HaeIII gg|cc
HincII gty|rac
HindIII a|agctt
HinfI g|antc
HpaI gtt|aac
HpaII c|cgg
KasI g|gcgcc
KpnI ggtac|c
MboI |gatc
MfeI c|aattg
MluI a|cgcgt
MscI tgg|cca
MseI t|taa
MspI c|cgg
NaeI gcc|ggc
NarI gg|cgcc
NcoI c|catgg
NdeI ca|tatg
NdeII |gatc
NgoMIV g|ccggc
NheI g|ctagc
NotI gc|ggccgc
NruI tcg|cga
NsiI atgca|t
PacI ttaat|taa
PciI a|catgt
PhoI gg|cc
PmeI gttt|aaac
PmlI cac|gtg
PsiI tta|taa
PstI ctgca|g
PvuI cgat|cg
PvuII cag|ctg
RsaI gt|ac
SacI gagct|c
SacII ccgc|gg
SalI g|tcgac
SbfI cctgca|gg
ScaI agt|act
SfoI ggc|gcc
SmaI ccc|ggg
SnaBI tac|gta
SpeI a|ctagt
SphI gcatg|c
SspI aat|att
SstI gagct|c
SstII ccgc|gg
StuI agg|cct
SwaI attt|aaat
TaqI t|cga
TliI c|tcgag
VspI at|taat
XbaI t|ctaga
XhoI c|tcgag
XmaI c|ccggg
sites.
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standard (1)
vertebrate mitochondrial (2)
yeast mitochondrial (3)
mold mitochondrial (4)
invertebrate mitochondrial (5)
ciliate nuclear (6)
echinoderm mitochondrial (9)
euplotid nuclear (10)
bacterial (11)
alternative yeast nuclear (12)
ascidian mitochondrial (13)
flatworm mitochondrial (14)
Blepharisma macronuclear (15)
chlorophycean mitochondrial (16)
trematode mitochondrial (21)
Scenedesmus obliquus mitochondrial (22)
Thraustochytrium mitochondrial (23)
genetic code
.